Review



primary rat aortic smooth muscle cells rasmcs  (Cell Applications Inc)


Bioz Verified Symbol Cell Applications Inc is a verified supplier
Bioz Manufacturer Symbol Cell Applications Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Cell Applications Inc primary rat aortic smooth muscle cells rasmcs
    Primary Rat Aortic Smooth Muscle Cells Rasmcs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/Rat+Smooth+Muscle+Cell+Media/pmc05129766-33-0-10
    Average 93 stars, based on 9 article reviews
    primary rat aortic smooth muscle cells rasmcs - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Platelet-derived growth factor-BB mediates cell migration through induction of activating transcription factor 4 and tenascin-C.
    Article Snippet: .. Primary rat aortic smooth muscle cells (RASMCs) were obtained from Cell Applications (San Diego, CA) and cultured in Waymouth’s medium, pH 7.4, containing 10% fetal bovine serum (FBS) and antibiotics as described previously.24 Cells were rendered quiescent by incubation in Waymouth’s medium without FBS for 24 hours. ..

    Incubation:

    Article Title: Platelet-derived growth factor-BB mediates cell migration through induction of activating transcription factor 4 and tenascin-C.
    Article Snippet: .. Primary rat aortic smooth muscle cells (RASMCs) were obtained from Cell Applications (San Diego, CA) and cultured in Waymouth’s medium, pH 7.4, containing 10% fetal bovine serum (FBS) and antibiotics as described previously.24 Cells were rendered quiescent by incubation in Waymouth’s medium without FBS for 24 hours. ..



    Similar Products

    primary rat aortic smooth muscle cells rasmcs  (Cell Applications Inc)
    93
    Cell Applications Inc primary rat aortic smooth muscle cells rasmcs
    Primary Rat Aortic Smooth Muscle Cells Rasmcs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/Rat+Smooth+Muscle+Cell+Media/pmc05129766-33-0-10
    Average 93 stars, based on 1 article reviews
    primary rat aortic smooth muscle cells rasmcs - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    primary rat aortic smooth muscle cells (rasmc)  (Dawley Inc)
    90
    Dawley Inc primary rat aortic smooth muscle cells (rasmc)
    Primary Rat Aortic Smooth Muscle Cells (Rasmc), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/rat+aortic+smooth+muscle+cells/pmc08145619-49-0-9
    Average 90 stars, based on 1 article reviews
    primary rat aortic smooth muscle cells (rasmc) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary rat aortic vascular smooth muscle cells (rasmc)  (Dawley Inc)
    90
    Dawley Inc primary rat aortic vascular smooth muscle cells (rasmc)
    Primary Rat Aortic Vascular Smooth Muscle Cells (Rasmc), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/primary+rat+aortic+vascular+smooth+muscle+cells++vsmcs+/pm32768401-89-0-11
    Average 90 stars, based on 1 article reviews
    primary rat aortic vascular smooth muscle cells (rasmc) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary rat aortic vascular smooth muscle cells (rasmcs)  (Dawley Inc)
    90
    Dawley Inc primary rat aortic vascular smooth muscle cells (rasmcs)
    Ang II induces autophagy in A7r5 and <t>RASMCs.</t> (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic <t>VSMCs</t> (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.
    Primary Rat Aortic Vascular Smooth Muscle Cells (Rasmcs), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/primary+rat+aortic+vascular+smooth+muscle+cells++vsmcs+/pmc06371839-38-0-11
    Average 90 stars, based on 1 article reviews
    primary rat aortic vascular smooth muscle cells (rasmcs) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary cultures of rat aortic smooth muscle cells (rasmcs)  (Vec Technologies)
    90
    Vec Technologies primary cultures of rat aortic smooth muscle cells (rasmcs)
    Ang II induces autophagy in A7r5 and <t>RASMCs.</t> (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic <t>VSMCs</t> (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.
    Primary Cultures Of Rat Aortic Smooth Muscle Cells (Rasmcs), supplied by Vec Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/rat+aortic+smooth+muscle+cells/pmc04860108-46-3-12
    Average 90 stars, based on 1 article reviews
    primary cultures of rat aortic smooth muscle cells (rasmcs) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary rat vascular aortic smooth muscle cells (rasmcs)  (Dawley Inc)
    90
    Dawley Inc primary rat vascular aortic smooth muscle cells (rasmcs)
    Ang II induces autophagy in A7r5 and <t>RASMCs.</t> (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic <t>VSMCs</t> (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.
    Primary Rat Vascular Aortic Smooth Muscle Cells (Rasmcs), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/primary+rat+aortic+vascular+smooth+muscle+cells++vsmcs+/10__1111_slash_ijs__12367-5150-0-17
    Average 90 stars, based on 1 article reviews
    primary rat vascular aortic smooth muscle cells (rasmcs) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    primary healthy rat aortic smooth muscle cells (rasmcs)  (Dawley Inc)
    90
    Dawley Inc primary healthy rat aortic smooth muscle cells (rasmcs)
    Ang II induces autophagy in A7r5 and <t>RASMCs.</t> (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic <t>VSMCs</t> (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.
    Primary Healthy Rat Aortic Smooth Muscle Cells (Rasmcs), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+aortic+smooth+muscle+cells+rasmcs/rat+aortic+smooth+muscle+cells/pmc03622827-69-0-17
    Average 90 stars, based on 1 article reviews
    primary healthy rat aortic smooth muscle cells (rasmcs) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Ang II induces autophagy in A7r5 and RASMCs. (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic VSMCs (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.

    Journal: Frontiers in Pharmacology

    Article Title: Angiotensin II-Regulated Autophagy Is Required for Vascular Smooth Muscle Cell Hypertrophy

    doi: 10.3389/fphar.2018.01553

    Figure Lengend Snippet: Ang II induces autophagy in A7r5 and RASMCs. (A) A7r5 cells were stimulated with Ang II 100 nM for 0, 0.5, 1, 3, 6, 12, 24, and 48 h (left panel) and with 1, 10, and 100 nM for 24 h, in presence and absence of CQ 30 μM, added for the last 4 h of stimulus (right panel). The LC3 II levels were determined by Western blot. The upper panels show the representative Western blots, whereas lower panels show the quantification of the LC3 II levels. β-Tubulin was used as loading control ( n = 4–5). (B) Primary cultures of rat aortic VSMCs (RASMCs) were stimulated with 100 nM of Ang II for 24 h in the presence and absence of CQ 30 μM, added during the last 4 h of stimulus. LC3 II levels and autophagic flux were determined by Western blot. β-Tubulin was used as loading control ( n = 4). (C) A7r5 cells were transduced with an adenovirus overexpressing LC3-GFP (ad-LC3-GFP), using a MOI of 180 and Hoechst as nuclear stain. After 24 h of incubation, cells were stimulated with 100 nM of Ang II for 24 h. During the last 4 h of stimulus, cells were then incubated in the presence or absence of 30 μM CQ. Representative images were obtained with a confocal microscope using a 40x lens and data are expressed percentage of autophagic cells ( n = 3, 30 cells per n). Scale bar = 25 μm. The results are shown as mean ± SEM. Data were analyzed using ANOVA. Newman–Keuls was used as post hoc test. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. control; ## p < 0.01, ### p < 0.001 vs. Ang II 100 nM, ωωω p < 0.001 vs. CQ.

    Article Snippet: Primary rat aortic vascular smooth muscle cells (RASMCs) were prepared from Sprague-Dawley rats (200–250 g).

    Techniques: Western Blot, Control, Transduction, Staining, Incubation, Microscopy